Packaging is important in preserving highly perishable fresh produce. Polymeric films commonly used in food packaging have low values of O2 and CO2 permeability. Previous attempts were made to study the O2 and CO2 permeability of micro-polymeric films as a function of size and diameter of perforations, but temperature of needle used for perforating the film could also affect the gas permeability of film. The objective of this work was to evaluate the effect of number, size perforation and temperature of needle on O2 and CO2 permeability of micro-perforated film. The number of perforations (2, 4, 6), size of perforation (0.25, 0.5, 0.85 mm) and needle temperature (10, 55, 100°C) were studied using a factorial design. Experiment set-up consisted of an airtight container with film at the top, which was initially flushed with an initial concentration of 0% O2 and 20% CO2, and the changes in O2 and CO2 concentrations were measured using gas analyser. Surface plots showed that permeability coefficient increased with size and number of perforations. Permeability coefficients for a film with 6 holes, diameter 0.25 mm, and needle temperature of 55°C were calculated for CO2 (1.66x10-8 m3/s) and O2 (2x10-8 m3/s), whereas without perforations permeability coefficients for CO2 and O2,, were 8.6x10-9 and 1x10-8 m3/s, respectively. At very low needle temperatures (10°C) the permeability coefficient is higher than at mid temperatures, but, above 80°C the permeability coefficient showed a slightly increased. In order to maximise mass transfer, packaging films should have several perforations with a large diameter and the needle temperature should be at 10 °C.