Peer-Reviewed Journal Details
Mandatory Fields
Lahart, Natasha and O'Callaghan, Yvonne and Aherne, S. Aisling and O'Sullivan, Dara and FitzGerald, Richard J. and O'Brien, Nora M.
International Dairy Journal
Extent of hydrolysis effects on casein hydrolysate bioactivity: Evaluation using the human Jurkat T cell line
Optional Fields
Two proteolytic preparations, A and F, identified in a previous study to yield casein hydrolysates with the highest bioactivity in Jurkat T cells, were used to hydrolyse sodium caseinate to determine if the bioactive effects of resultant hydrolysates (A1-A5 and F1-F5) were influenced by degree of hydrolysis (DH). None of the hydrolysates (0.25-10.0%, v/v) adversely affected membrane integrity. However, A4 significantly (P< 0.05) reduced Jurkat T cell viability at all concentrations and F5 decreased cell viability at concentrations exceeding 2.50% (v/v). All A hydrolysates, except A4, significantly affected both glutathione (GSH) content and cellular catalase activity at 0.5% (v/v). The effect of F hydrolysates on GSH content were variable. All samples apart from A4 significantly increased catalase activity. None of the casein hydrolysates significantly affected (P < 0.05) ConA-stimulated interleukin-2 production; however, A2 and A3 increased interleukin-10 production. While hydrolysis is considered necessary to release bioactive peptides, no direct bioactivity-DH relationship was observed. (C) 2011 Elsevier Ltd. All rights reserved.
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