Peer-Reviewed Journal Details
Mandatory Fields
Tyther, R., McDonagh, B. and Sheehan D
Mass Spectrometry Reviews
Proteomics in investigation of protein nitration in kidney disease: Technical challenges and perspectives from the spontaneously hypertensive rat.
Optional Fields

Kidneys are the mammalian organs with widest range of

oxidative status ranging from the well-perfused cortex to the

relatively anoxic medulla. This organ is of key interest from the

perspective of hypertension, an important contributor to human

mortality, and there has been growing use of the spontaneously

hypertensive rat (SHR) as a model to explore oxidative stress in

hypertensive kidney. Nitrosative stress is often associated with

oxidative stress and, like oxidative stress, can lead to covalent

modification of protein side-chains. It is especially relevant to

kidney because of high levels of both nitrite/nitrate and nitric

oxide synthase in medulla. Because of their relatively low

abundance and their well-known role in signal transduction,

nitration of tyrosines to 3-nitrotyrosines (3NT) is of particular

interest in this regard. This modification has the potential to

contribute to changes in regulation, in protein activity and may

provide a means of specific targeting of key proteins. Mass

spectrometry (MS) offers a promising route to detecting this

modification. This review surveys protein nitration in kidney

disease and highlights opportunities for MS detection of

nitrated residues in the SHR.

Grant Details