Peer-Reviewed Journal Details
Mandatory Fields
Mues, A.,van der Ven, P. F.,Young, P.,Furst, D. O.,Gautel, M.;
1998
Two immunoglobulin-like domains of the Z-disc portion of titin interact in a conformation-dependent way with telethonin
Validated
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Optional Fields
428
1-2
111
4.
The giant muscle protein titin/connectin plays a crucial role in myofibrillogenesis as a molecular ruler for sarcomeric protein sorting. We describe here that the N-terminal titin immunoglobulin domains Z1 and Z2 interact specifically with telethonin in yeast two-hybrid analysis and protein binding assays. Immunofluorescence with antibodies against the N-terminal region of titin and telethonin detects both proteins at the Z-disc of human myotubes. Longer titin fragments, comprising a serine-proline-rich phosphorylation site and the next domain, do not interact. The interaction of telethonin with titin is therefore conformation-dependent, reflecting a possible phosphorylation regulation during myofibrillogenesis.
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?db=m&form=6&dopt=r&uid=9645487
Grant Details