Peer-Reviewed Journal Details
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Smith, L. M.,Walsh, P. T.,Rudiger, T.,Cotter, T. G.,Mc Carthy, T. V.,Marx, A.,O'Connor, R.;
Experimental Cell Research
EphA3 is induced by CD28 and 1GF-1 and regulates cell adhesion
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Stimulation of CD28 alone has been shown to regulate cytokine gene transcription and expression of the type I insulin-like growth factor receptor (IGF-lR) in lymphocytes. In this study, the ephrin receptor tyrosine kinase ephA3, was identified as a new CD28-responsive gene in Jurkat cells by using a human cytokine/receptor array. EphA3 was not detected in normal peripheral T cells, in any subset of thymus-derived developing T cells, or in Hodgkin's lymphoma. However, contrary to previous findings, EphA3 was detected in a panel of T-cell lymphomas. Stimulation of Jurkat cells with ephrin-A5 resulted in loss of cell adhesion to fibronectin and recruitment of the adapter protein CrkII to EphA3. Interestingly, EphA3 expression in CD28-stimulated Jurkat cells was enhanced by IGF-1 or by overexpression of the IGF-IR, and was suppressed by anti-IGF-IR blocking antibodies. The data suggest that CD28- and IGF-1- regulated expression of EphA3 is associated with adherence and that it may be involved in the motility of malignant T cells. (C) 2003 Elsevier Inc. All rights reserved.
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