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Koren, K,Dmitriev, RI,Borisov, SM,Papkovsky, DB,Klimant, I
2012
January
Chembiochem
Complexes of IrIII-Octaethylporphyrin with Peptides as Probes for Sensing Cellular O2
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cell-penetrating peptide cellular oxygen sensing histidine ligands Ir-porphyrin complexes phosphorescence quenching OXYGEN-SENSITIVE PROBES PENETRATING PEPTIDES INTRACELLULAR OXYGEN IN-VIVO INTERNALIZATION MECHANISMS PHOSPHORESCENT PROBES METABOLIC-RESPONSES PROTOPORPHYRIN-IX MAMMALIAN-CELLS LIVE CELLS
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IrIIIporphyrins are a relatively new group of phosphorescent dyes that have potential for oxygen sensing and labeling of biomolecules. The requirement of two axial ligands for the IrIII ion permits simple linkage of biomolecules by a one-step ligand-exchange reaction, for example, using precursor carbonyl chloride complexes and peptides containing histidine residue(s). Using this approach, we produced three complexes of IrIIIoctaethylporphyrin with cell-penetrating (Ir1 and Ir2) and tumor-targeting (Ir3) peptides and studied their photophysical properties. All of the complexes were stable and possessed bright, long-decay (unquenched lifetimes exceeding 45 mu s) phosphorescence at around 650 nm, with moderate sensitivity to oxygen. The Ir1 and Ir2 complexes showed positive staining of a number of mammalian cell types, thus demonstrating localization similar to endoplasmic reticulum and ATP- and temperature-independent intracellular accumulation (direct translocation mechanism). Their low photo- and cytotoxicity allows intracellular oxygen to be probed.
DOI 10.1002/cbic.201200083
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