Peer-Reviewed Journal Details
Mandatory Fields
Suda, S,Westerbeek, A,O'Connor, PM,Ross, RP,Hill, C,Cotter, PD
2010
January
Chemistry & Biology
Effect of Bioengineering Lacticin 3147 Lanthionine Bridges on Specific Activity and Resistance to Heat and Protease's
Validated
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Optional Fields
2-COMPONENT LANTIBIOTIC LACTICIN-3147 PRECURSOR LIPID-II STAPHYLOCOCCUS-AUREUS ANTIMICROBIAL ACTIVITY BIOLOGICAL-ACTIVITY LACTOCOCCUS-LACTIS THIOETHER BRIDGE NISIN PEPTIDES BIOSYNTHESIS
17
1151
1160
Lacticin 3147 is a lantibiotic with seven lanthionine bridges across its two component peptides, Ltn alpha and Ltn beta Although it has been proposed that the eponymous lanthionine and (beta-methyl)lanthionine (Lan and meLan) bridges present in lantibiotics make an important contribution to protecting the peptides from thermal or proteolytic degradation, few studies have investigated this link We have generated a bank of bioengineered derivatives of lacticin 3147, in which selected bridges were removed or converted between Lan and meLan, which were exposed to high temperature or proteolytic enzymes Although switching Lan and meLan bridges has variable consequences, it was consistently observed that an intact N-terminal lanthionine bridge (Ring A) confers Ltna with enhanced resistance to thermal and proteolytic degradation
DOI 10.1016/j.chembiol.2010.08.011
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