Laing, AJ,Dillon, JP,Condon, ET,Street, JT,Wang, JH,McGuinness, AJ,Redmond, HP

2007

January

Journal Of Orthopaedic Research

Mobilization of endothelial precursor cells: Systemic vascular response to musculoskeletal trauma

Validated

()

endothelial precursor cells neovascularization vasculogenesis PROGENITOR CELLS EX-VIVO POSTNATAL NEOVASCULARIZATION ISCHEMIC MYOCARDIUM GENE-TRANSFER ANGIOGENESIS BLOOD ANGIOBLASTS MICE VASCULOGENESIS

25

44

50

Postnatal vasculogenesis, the process by which vascular committed bone marrow stem cells or endothelial precursor cells (EPC) migrate, differentiate, and incorporate into the nacent endothelium contributing to physiological and pathological neovascularization, has stimulated much interest. Its contribution to tumor nonvascularization, wound healing, and revascularization associated with skeletal and cardiac muscles ischaernia is established. We evaluated the mobilization of EPCs in response to musculoskeletal trauma. Blood from patients (n = 15) following AO type 42a1 closed diaphyseal tibial fractures was analyzed for CD34 and AC133 cell surface marker expression. Immunomagnetically enriched CD34+ mononuclear cell (MNCCD34+) populations were cultured and examined for phenotypic and functional vascular endothelial differentiation. Circulating MNCCD34+, levels increased sevenfold by day 3 postinjury. Circulating MNCAC133+ increased 2.5-fold. Enriched MNCCD34+ populations from day 3 samples in culture exhibited cell cluster formation with sprouting spindles. These cells bound UEA-1 and incorporated fluorescent DiI-Ac-LDL intracellularily. Our findings suggest a systemic provascular response is initiated in response to musculoskeletal trauma. Its therapeutic manipulation may have implications for the potential enhancement of fracture healing. (c) 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

DOI 10.1002/jor.20228