Cotter, PD,Draper, LA,Lawton, EM,McAuliffe, O,Hill, C,Ross, RP

2006

June

Applied and Environmental Microbiology

Overproduction of wild-type and bioengineered derivatives of the lantibiotic lacticin 3147

Validated

()

LACTIS SUBSP LACTIS RESISTANT STAPHYLOCOCCUS-AUREUS LACTOCOCCUS-LACTIS GENE-CLUSTER BIOSYNTHESIS IMMUNITY NISIN BACTERIOCIN EXPRESSION IDENTIFICATION

72

4492

4496

Lacticin 3147 is a broad-spectrum two-peptide lantibiotic whose genetic determinants are located on two divergent operons on the lactococcal plasmid pMRC01. Here we introduce each of 14 subclones, containing different combinations of lacticin 3147 genes, into MG1363 (pMRC01) and determine that a number of them can facilitate overproduction of the lantibiotic. Based on these studies it is apparent that while the provision of additional copies of genes encoding the biosynthetic/production machinery and the regulator LtnR is a requirement for high-level overproduction, the presence of additional copies of the structural genes (i.e., ltnA1A2) is not.

DOI 10.1128/AEM.02543-05