Peer-Reviewed Journal Details
Mandatory Fields
Kennedy, J,O'Leary, ND,Kiran, GS,Morrissey, JP,O'Gara, F,Selvin, J,Dobson, ADW
2011
January
Journal of Applied Microbiology
Functional metagenomic strategies for the discovery of novel enzymes and biosurfactants with biotechnological applications from marine ecosystems
Validated
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Optional Fields
biotechnology biosurfactants cellulases laccases lipases metagenomics oxidoreductases proteases SOUTH CHINA SEA UNCULTURED MICROORGANISMS MARINOMONAS-MEDITERRANEA MICROBIAL SURFACTANTS EXPRESSION CLONING DIRECTED EVOLUTION ESCHERICHIA-COLI BOVINE RUMEN BACILLUS SP LIBRARIES
111
787
799
Marine ecosystems are home to bacteria which are exposed to a wide variety of environmental conditions, such as extremes in temperature, salinity, nutrient availability and pressure. Survival under these conditions must have necessitated the adaptation and the development of unique cellular biochemistry and metabolism by these microbes. Thus, enzymes isolated from these microbes have the potential to possess quite unique physiological and biochemical properties. This review outlines a number of function-based metagenomic approaches which are available to screen metagenomic libraries constructed from marine ecosystems to facilitate the exploitation of some of these potentially novel biocatalysts. Functional screens to isolate novel cellulases, lipases and esterases, proteases, laccases, oxidoreductases and biosurfactants are described, together with approaches which can be employed to help overcome some of the typical problems encountered with functional metagenomic-based screens.
DOI 10.1111/j.1365-2672.2011.05106.x
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