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Field, D,Hill, C,Cotter, PD,Ross, RP
2010
January
The dawning of a 'Golden era' in lantibiotic bioengineering
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RESISTANT STAPHYLOCOCCUS-AUREUS NONPROTEINOGENIC AMINO-ACIDS ANTIMICROBIAL PEPTIDE NISIN GENE REPLACEMENT STRATEGY SITE-DIRECTED MUTAGENESIS IN-VITRO RECONSTITUTION PRECURSOR LIPID-II LACTOCOCCUS-LACTIS POSTTRANSLATIONAL MODIFICATION EXPRESSION SYSTEM
P>There are many examples of highly modified antimicrobial peptides in nature, many of which are non-ribosomally synthesized. However, the bacterial lantibiotics are produced as gene-encoded pre-peptides that are subsequently modified by dedicated enzyme systems to form extraordinarily potent inhibitors. Consequently, they are much more amenable to bioengineering which could lead to the generation of a new arsenal of potent antimicrobials. However, although bioengineering of these compounds has been underway for at least two decades, significant progress has only been reported in recent years. This review charts these recent developments which suggest that we are entering a 'Golden era' of lantibiotic bioengineering.
1077
1087
DOI 10.1111/j.1365-2958.2010.07406.x
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