Moore, E,Sanvicens, N,Pravda, M,Guilbault, GG

2003

March

International Journal of Environmental Analytical Chemistry

Practical study into enhancing IgG immobilisation on screen-printed electrodes for environmental applications

Validated

()

immunosensor immobilisation screen-printed electrode antibody orientation IgG biosensors alkaline phosphatase p-aminophenyl phosphate ANTIBODY IMMOBILIZATION ORIENTED IMMOBILIZATION CARBON-PASTE ORIENTATION ENZYMES SENSOR FILMS WINE

83

545

553

This work is focused on the influence of pH and applied potential for the immobilisation of rabbit IgG on a carbon screen-printed electrode. The orientation of IgG molecule at the surface is fundamental for activity and reproducibility of the immunosensor. As the electrode potential is increased a particular order may be brought to the immobilised antibodies, i.e. the antibodies adapt a more favourable arrangement on the surface to facilitate better binding. The response increased when changing the pH from basic to acidic medium and the reverse trend was observed for the limit of detection (LOD). When a potential was applied to the electrode, the response generally decreased and the LOD increased in the order acidic > basic > neutral pH. The LODs obtained from antibodies immobilised at acidic pH and + 100 mV were better than the LODs obtained at other conditions.

DOI 10.1080/0306731021000019197