Skolimowski, M,Nielsen, MW,Abeille, F,Skafte-Pedersen, P,Sabourin, D,Fercher, A,Papkovsky, D,Molin, S,Taboryski, R,Sternberg, C,Dufva, M,Geschke, O,Emneus, J

2012

January

Biomicrofluidics

Modular microfluidic system as a model of cystic fibrosis airways

Validated

()

CHRONIC LUNG INFECTION PSEUDOMONAS-AERUGINOSA ENDOBRONCHITIS BLOOD-BRAIN-BARRIER IN-VITRO MODEL CELL-CULTURE METABOLIC-RESPONSES BIOFILM FORMATION MAMMALIAN-CELLS MAXILLARY SINUS RHESUS-MONKEYS

6

A modular microfluidic airways model system that can simulate the changes in oxygen tension in different compartments of the cystic fibrosis (CF) airways was designed, developed, and tested. The fully reconfigurable system composed of modules with different functionalities: multichannel peristaltic pumps, bubble traps, gas exchange chip, and cell culture chambers. We have successfully applied this system for studying the antibiotic therapy of Pseudomonas aeruginosa, the bacteria mainly responsible for morbidity and mortality in cystic fibrosis, in different oxygen environments. Furthermore, we have mimicked the bacterial reinoculation of the aerobic compartments (lower respiratory tract) from the anaerobic compartments (cystic fibrosis sinuses) following an antibiotic treatment. This effect is hypothesised as the one on the main reasons for recurrent lung infections in cystic fibrosis patients. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4742911]

ARTN 034109