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Watson, D.,Motherway, M. O.,Schoterman, M. H.,van Neerven, R. J.,Nauta, A.,van Sinderen, D.
2012
December
Selective carbohydrate utilization by lactobacilli and bifidobacteria
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AIM: To evaluate the ability of specific carbohydrates, including commercially available products, to support growth of representatives of two well-known groups of gut commensals, namely lactobacilli and bifidobacteria. METHODS AND RESULTS: Sixty-eight bacterial strains, representing twenty-nine human-derived lactobacilli and thirty-nine bifidobacteria (both human- and animal-derived), were tested for their ability to metabolize ten different carbohydrates. Analysis of growth and metabolic activity was performed using a combination of diagnostic parameters, such as final OD(600) , final pH, fermentation end products and growth rate. CONCLUSIONS: The data assembled in this study provide significant complementary and comparative information on the growth-promoting properties of a range of carbohydrates, while also investigating inter-species differences between lactobacilli and/or bifidobacteria with regards to their carbohydrate utilization abilities. GOS and lactulose were shown to support the most favourable growth characteristics, whereas relatively poor growth of lactobacilli and bifidobacteria was observed on inulin, maltodextrin and polydextrose. GOS/inulin (9:1) and FOS/inulin mixtures supported mostly similar growth abilities to those obtained for GOS and FOS, respectively. Microbial consumption of GOS, as determined by HPAEC-PAD, was evident for both lactobacilli and bifidobacteria. SIGNIFICANCE AND IMPACT OF THE STUDY: These results may allow for the rational prediction of lactobacilli and/or bifidobacteria to be used in conjunction with prebiotics, such as GOS, as synbiotics. (c)2012 University College Cork.AIM: To evaluate the ability of specific carbohydrates, including commercially available products, to support growth of representatives of two well-known groups of gut commensals, namely lactobacilli and bifidobacteria. METHODS AND RESULTS: Sixty-eight bacterial strains, representing twenty-nine human-derived lactobacilli and thirty-nine bifidobacteria (both human- and animal-derived), were tested for their ability to metabolize ten different carbohydrates. Analysis of growth and metabolic activity was performed using a combination of diagnostic parameters, such as final OD(600) , final pH, fermentation end products and growth rate. CONCLUSIONS: The data assembled in this study provide significant complementary and comparative information on the growth-promoting properties of a range of carbohydrates, while also investigating inter-species differences between lactobacilli and/or bifidobacteria with regards to their carbohydrate utilization abilities. GOS and lactulose were shown to support the most favourable growth characteristics, whereas relatively poor growth of lactobacilli and bifidobacteria was observed on inulin, maltodextrin and polydextrose. GOS/inulin (9:1) and FOS/inulin mixtures supported mostly similar growth abilities to those obtained for GOS and FOS, respectively. Microbial consumption of GOS, as determined by HPAEC-PAD, was evident for both lactobacilli and bifidobacteria. SIGNIFICANCE AND IMPACT OF THE STUDY: These results may allow for the rational prediction of lactobacilli and/or bifidobacteria to be used in conjunction with prebiotics, such as GOS, as synbiotics. (c)2012 University College Cork.
1365-2672 (Electronic) 13
http://www.ncbi.nlm.nih.gov/pubmed/23240984http://www.ncbi.nlm.nih.gov/pubmed/23240984
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