Peer-Reviewed Journal Details
Mandatory Fields
Sarah J. Brooks, Jasmina Nikodinovic, Leona Martin, Evelyn M. Doyle, Timothy O’Sullivan, Patrick J. Guiry, Lydie Coulombel, Zhi Li, Kevin E. O’Connor
Biotechnology Letters
Production of a chiral alcohol, 1-(3,4-dihydroxyphenyl)
Optional Fields
Tyrosinase  Biotransformation Oxidation Chiral product

1-(3,4-Dihydroxyphenyl) ethanol was produced biocatalytically for the first time using mushroom tyrosinase. 4-Ethylphenol at 1 mMwas consumed over 12 min giving 0.23 mM 4-ethylcatechol and 0.36 mM (R/S)-1-(3,4-dihydroxyphenyl) ethanol (ee 0.5 %). Mushroom tyrosinase consumed 4-ethylphenol at 6.7 lmol min-1 mg protein-1 while the rates of formation of 4-ethylcatechol and 1-(3,4-dihydroxyphenyl) ethanol were 1.1 and 1.9 lmol min-1 mg protein-1. Addition of the ascorbic acid, as a reducing agent to biotransformation reactions, increased 4-ethylcatechol formation by 340 %. However, accumulation of 1-(3,4-dihydroxyphenyl) ethanol was not observed in the presence of ascorbic acid. While the 1-(3,4-dihydroxyphenyl) ethanol was racemic, it is the first chiral product produced by tyrosinase starting from a non-chiral substrate.

Grant Details