Denihan, NM,Looney, AM,Boylan, GB,Walsh, BH,Murray, DM

2013

December

Clinical biochemistry

Normative levels of Interleukin 16 in umbilical cord blood

Validated

()

Interleukin 16 Hypoxic-ischaemic encephalopathy Umbilical cord blood Cytokines Vaginal delivery Elective caesarean section Pregnancy ELISA ZEALAND SOCIOECONOMIC INDEX TUMOR-NECROSIS-FACTOR DELIVERY IL-16 EXPRESSION PREECLAMPSIA MONOCYTES MODE

46

1857

1859

Objectives: The need for early and accurate prediction of outcome in hypoxic-ischaemic encephalopathy (HIE) remains critical. We have previously demonstrated that Interleulkin 16 (IL-16) is raised in the umbilical cord blood (UCB) of infants with moderate and severe HIE and has the potential to be developed as a predictive biomarker. Normal reference ranges for IL-16 in UCB have not been previously described. The aim of this study was to determine normative levels of IL-16 in full term neonates using UCB following uncomplicated deliveries and to examine the effect of labour on cord IL-16 values.Design and methods: Full term infants were recruited as part of an ongoing birth cohort study, the Cork BASELINE Birth Cohort Study. All had UCB drawn and bio-banked at - 80 degrees C, within 3 hours of birth. Samples for this experiment were chosen from this population based cohort study to represent uncomplicated pre-labour caesarean sections and spontaneous vaginal deliveries. Analysis was performed on plasma EDTA, using ELISA Quantikine (R) (R&D Systems, Europe).Results: Samples were analysed from 48 infants with two modes of delivery; spontaneous vaginal delivery (n = 12 male, n = 12 female) and elective caesarean section (n = 12 male, n = 12 female). The range of all samples was normally distributed between 87.0 and 114.6 pg/ml. Overall mean (SD) for IL-16 was 102.9 (21.5) pg/ml. Levels were not affected by spontaneous vaginal delivery or gender.Conclusion: For the first time we have described the expected range of cord plasma IL-16 levels in healthy term infants following pre-labour and post-labour delivery. (C) 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

10.1016/j.clinbiochem.2013.07.012