Peer-Reviewed Journal Details
Mandatory Fields
Scannell, AGM,Hill, C,Ross, RP,Marx, S,Hartmeier, W,Arendt, EK
2000
October
Journal of Applied Microbiology
Continuous production of lacticin 3147 and nisin using cells immobilized in calcium alginate
Validated
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Optional Fields
PACKED-BED BIOREACTOR BACTERIOCIN PRODUCTION PEDIOCIN ACH LISTERIA-MONOCYTOGENES LACTOBACILLUS-CASEI PLASMID STABILITY ACID BACTERIA INHIBITION DIFFUSION WHEY
89
573
579
Bacteriocinogenic strains, Lactococcus lactis subsp. lactis DPC 3147 and L. lactis DPC 496, producing lacticin 3147 and nisin, respectively, were immobilized in double-layered calcium alginate beads. These beads were inoculated into MRS broth at a ratio of 1:4 and continuously fermented for 180 h. Free cells were used to compare the effect of immobilization on bacteriocin production. After equilibrium was reached, a flow rate of 580 ml h(-1) was used in the immobilized cell (IC), and 240 ml h(-1) in free-cell (FC) bioreactors. Outgrowth from beads was observed after 18 h. Bacteriocin production peaked at 5120 AU ml(-1) in both IC and FC bioreactors. However, FC production declined after 80 h to 160 AU ml(-1) at the end of the fermentation. Results of this study indicate that immobilization offers the possibility of a more stable and long-term means of producing lacticin 3147 in laboratory media than with free cells.
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