Peer-Reviewed Journal Details
Mandatory Fields
Nishihara, T,Morisawa, H,Ohta, N,Atkins, JF,Nishimura, Y
2004
October
Genes To Cells
A cryptic lysis gene near the start of the Q beta replicase gene in the+1 frame
Validated
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Optional Fields
COMPLETE NUCLEOTIDE-SEQUENCE PROGRAMMED TRANSLATIONAL FRAMESHIFT SECONDARY STRUCTURE MODEL ESCHERICHIA-COLI LYSIS BACTERIOPHAGE-Q-BETA COLIPHAGE-Q-BETA PHAGE Q-BETA COAT PROTEIN CELL-LYSIS RNA PHAGE
9
877
889
The maturation/lysis (A2) protein encoded by the group B single-stranded RNA bacteriophage Qbeta mediates lysis of host Escherichia coli cells. We found a frameshift mutation in the replicase (beta-subunit) gene of Qbeta cDNA causes cell lysis. The mutant has a single base deletion 73 nucleotides (nt) 3' from the start of the replicase gene with consequent translation termination at a stop codon 129-131 nt further 3'. The 43-amino acid C-terminal part of the 67-amino acid product encoded by what in WT (wild-type) is the +1 frame, is rich in basic amino acids This 67-aa protein can mediate cell lysis whose characteristics indicate that the protein may cause lysis by a different mechanism and via a different target, than that caused by the A2 maturation/lysis protein. Synthesis of a counterpart of the newly discovered lysis product in wild-type phage infection would require a hypothetical ribosomal frameshifting event. The lysis gene of group A RNA phages is also short, 75 codons in MS2, and partially overlaps the first part of their equivalently located replicase gene, raising significant evolutionary implications for the present finding.
10.1111/j.1365-2443.2004.00778.x
Grant Details