Peer-Reviewed Journal Details
Mandatory Fields
Milani, C,Hevia, A,Foroni, E,Duranti, S,Turroni, F,Lugli, GA,Sanchez, B,Martin, R,Gueimonde, M,van Sinderen, D,Margolles, A,Ventura, M
2013
July
Plos One
Assessing the Fecal Microbiota: An Optimized Ion Torrent 16S rRNA Gene-Based Analysis Protocol
Validated
Optional Fields
HUMAN INTESTINAL-TRACT GUT MICROBIOTA PHYLOGENETIC MICROARRAY BACTERIAL DIVERSITY COMMUNITIES SEQUENCE SUCCESSION DISEASE HEALTH
8
Assessing the distribution of 16S rRNA gene sequences within a biological sample represents the current state-of-the-art for determination of human gut microbiota composition. Advances in dissecting the microbial biodiversity of this ecosystem have very much been dependent on the development of novel high-throughput DNA sequencing technologies, like the Ion Torrent. However, the precise representation of this bacterial community may be affected by the protocols used for DNA extraction as well as by the PCR primers employed in the amplification reaction. Here, we describe an optimized protocol for 16S rRNA gene-based profiling of the fecal microbiota.
10.1371/journal.pone.0068739
Grant Details