Peer-Reviewed Journal Details
Mandatory Fields
Turroni, F,Serafini, F,Foroni, E,Duranti, S,Motherway, MO,Taverniti, V,Mangifesta, M,Milani, C,Viappiani, A,Roversi, T,Sanchez, B,Santoni, A,Gioiosa, L,Ferrarini, A,Delledonne, M,Margolles, A,Piazza, L,Palanza, P,Bolchi, A,Guglielmetti, S,van Sinderen, D,Ventura, M
2013
July
Proceedings of The National Academy of Sciences of The United States of America
Role of sortase-dependent pili of Bifidobacterium bifidum PRL2010 in modulating bacterium-host interactions
Validated
Optional Fields
genomics host-microbe interaction probiotics transcriptomics GRAM-POSITIVE BACTERIA EXTRACELLULAR-MATRIX PROTEINS INTESTINAL EPITHELIAL-CELLS LONG POLAR FIMBRIAE ESCHERICHIA-COLI STAPHYLOCOCCUS-AUREUS BREVE UCC2003 GENUS BIFIDOBACTERIUM MICROBE INTERACTIONS BIOFILM DEVELOPMENT
110
11151
11156
Bifidobacteria represent one of the dominant groups of microorganisms colonizing the human infant intestine. Commensal bacteria that interact with a eukaryotic host are believed to express adhesive molecules on their cell surface that bind to specific host cell receptors or soluble macromolecules. Whole-genome transcription profiling of Bifidobacterium bifidum PRL2010, a strain isolated from infant stool, revealed a small number of commonly expressed extracellular proteins, among which were genes that specify sortase-dependent pili. Expression of the coding sequences of these B. bifidum PRL2010 appendages in nonpiliated Lactococcus lactis enhanced adherence to human enterocytes through extracellular matrix protein and bacterial aggregation. Furthermore, such piliated L. lactis cells evoked a higher TNF-alpha response during murine colonization compared with their nonpiliated parent, suggesting that bifidobacterial sortase-dependent pili not only contribute to adherence but also display immunomodulatory activity.
10.1073/pnas.1303897110
Grant Details