Peer-Reviewed Journal Details
Mandatory Fields
O'Sullivan, O,Coakley, M,Lakshminarayanan, B,Claesson, MJ,Stanton, C,O'Toole, PW,Ross, RP,ELDERMET Consortium
2011
August
Journal of Applied Microbiology
Correlation of rRNA gene amplicon pyrosequencing and bacterial culture for microbial compositional analysis of faecal samples from elderly Irish subjects
Validated
WOS: 17 ()
Optional Fields
bacterial culture bifidobacteria elderly Irish lactobacilli sequencing HUMAN INTESTINAL MICROBIOTA GUT MICROBIOTA BIFIDOBACTERIA DIVERSITY AGE COMMUNITIES FLORA SUPPLEMENTATION IDENTIFICATION LACTOBACILLUS
111
467
473
Aims: The aim of this investigation was to establish the degree of correlation between measurements from culture-dependent microbiological techniques and from next generation sequencing technologies.Methods and Results: Data generated by both techniques were collected from faecal samples from 185 elderly Irish people involved in the ongoing ELDER-MET study (http://eldermet.ucc.ie). The results for three groups of intestinal bacteria were compared. Bifidobacterium sp., Lactobacillus sp. and Enterobacteriaceae were enumerated on selective media through culture-dependent techniques, whereas proportions of these bacteria were determined through sequencing technology against the background of other bacteria. The Spearman's rank correlation coefficient determined a good correlation between results from culture-dependent microbiology and culture-independent techniques for all three bacterial groups assessed (correlation coefficients for Bifidobacterium sp., Lactobacillus sp. and Enterobacteriaceae were 0.380, 0.366 and 0.437, respectively).Conclusion: Correlation between the two methods implies that a single method is capable of profiling intestinal Bifidobacterium, Lactobacillus and Enterobacteriaceae populations. However, both methods have advantages that justify their use in tandem.Significance and Impact of the Study: This is the first extensive study to compare bacterial counts from culture-dependent microbiological techniques and from next generation sequencing technologies.
10.1111/j.1365-2672.2011.05067.x
Grant Details