Peer-Reviewed Journal Details
Mandatory Fields
Riza, Nabeel A.; Sheikh, Mumtaz; Webb-Wood, Grady; Kik, Pieter G.
Optical Engineering
Demonstration of three-dimensional optical imaging using a confocal microscope based on a liquid-crystal electronic lens
Optional Fields
Liquid crystal Optical imaging Microscopy
Three-dimensional (3-D) imaging is demonstrated using an electronically controlled liquid crystal (LC) optical lens to accomplish a no-moving-parts depth-section scanning in a modified commercial 3-D confocal microscope. Specifically, 3-D views of a standard CDC blood vessel (enclosed in a glass slide) have been obtained using the modified confocal microscope operating at the red 633-nm laser wavelength. The image sizes over a 25-μm axial scan depth were 5050 μm and 8080 μm, using 60 and 20 micro-objectives, respectively. The transverse motion step was 0.1 μm for the 60 data and 0.2 μm for the 20 data. As a first-step comparison, image processing of the standard and LC electronic-lens microscope images indicates correlation values between 0.81 and 0.91. The proposed microscopy system within aberration limits has the potential to eliminate the mechanical forces due to sample or objective motion that can distort the original sample structure and lead to imaging errors.
Grant Details