Peer-Reviewed Journal Details
Mandatory Fields
Motherway, MO,Watson, D,Bottacini, F,Clark, TA,Roberts, RJ,Korlach, J,Garault, P,Chervaux, C,Vlieg, JETV,Smokvina, T,van Sinderen, D
2014
April
Plos One
Identification of Restriction-Modification Systems of Bifidobacterium animalis subsp lactis CNCM I-2494 by SMRT Sequencing and Associated Methylome Analysis
Validated
Optional Fields
FERMENTED MILK PRODUCT IMPROVED CLONING VECTORS BREVE UCC2003 LACTOCOCCUS-LACTIS ESCHERICHIA-COLI ANABAENA-VARIABILIS FUNCTIONAL-ANALYSIS GENE-EXPRESSION DN-173 010 GENOME
9
Bifidobacterium animalis subsp. lactis CNCM I-2494 is a component of a commercialized fermented dairy product for which beneficial effects on health has been studied by clinical and preclinical trials. To date little is known about the molecular mechanisms that could explain the beneficial effects that bifidobacteria impart to the host. Restriction-modification (R-M) systems have been identified as key obstacles in the genetic accessibility of bifidobacteria, and circumventing these is a prerequisite to attaining a fundamental understanding of bifidobacterial attributes, including the genes that are responsible for health-promoting properties of this clinically and industrially important group of bacteria. The complete genome sequence of B. animalis subsp. lactis CNCM I-2494 is predicted to harbour the genetic determinants for two type II R-M systems, designated BanLI and BanLII. In order to investigate the functionality and specificity of these two putative R-M systems in B. animalis subsp. lactis CNCM I-2494, we employed PacBio SMRT sequencing with associated methylome analysis. In addition, the contribution of the identified R-M systems to the genetic accessibility of this strain was assessed.
10.1371/journal.pone.0094875
Grant Details