Peer-Reviewed Journal Details
Mandatory Fields
Mathur, H,O'Connor, PM,Cotter, PD,Hill, C,Ross, RP
2014
June
Antimicrobial Agents and Chemotherapy
Heterologous Expression of Thuricin CD Immunity Genes in Listeria monocytogenes
Validated
WOS: 3 ()
Optional Fields
SECONDARY STRUCTURE PREDICTION MEMBRANE-PROTEIN TOPOLOGY LIPID-FREE NISI LACTOCOCCUS-LACTIS CLOSTRIDIUM-DIFFICILE PROPIONIBACTERIUM-FREUDENREICHII CONSENSUS PREDICTION LANTIBIOTIC IMMUNITY BACILLUS-SUBTILIS NARROW-SPECTRUM
58
3421
3428
Bacteriocins are ribosomally synthesized peptides that can have a narrow or broad spectrum of antimicrobial activity. Bacteriocin producers typically possess dedicated immunity systems that often consist of an ATP-binding cassette (ABC) transporter system and/or a dedicated immunity protein. Here we investigated the genes responsible for immunity to thuricin CD, a narrow-spectrum two-peptide sactibiotic produced by Bacillus thuringiensis DPC6431. Heterologous expression of putative thuricin CD immunity determinants allowed us to identify and investigate the relative importance of the individual genes and gene products that contribute to thuricin CD immunity. We established that TrnF and TrnG are the individual components of an ABC transporter system that provides immunity to thuricin CD. We also identified a hitherto overlooked open reading frame located upstream of trnF predicted to encode a 79-amino-acid transmembrane protein. We designated this newly discovered gene trnI and established that TrnI alone can provide protection against thuricin CD.
10.1128/AAC.00090-14
Grant Details