Peer-Reviewed Journal Details
Mandatory Fields
O'Mahony, AM,Cronin, MF,Mcmahon, A,Evans, JC,Daly, K,Darcy, R,O'Driscoll, CM
2014
May
Journal of Pharmaceutical Sciences
Biophysical and Structural Characterisation of Nucleic Acid Complexes with Modified Cyclodextrins Using Circular Dichroism
Validated
WOS: 12 ()
Optional Fields
circular dichroism plasmid DNA siRNA biopharmaceuticals characterization non-viral gene delivery NEURONAL SIRNA DELIVERY GENE DELIVERY CATIONIC LIPOSOMES DNA NANOPARTICLES TRANSFECTION VECTORS HEPATOCYTES TRAFFICKING PEGYLATION
103
1346
1355
Modified cyclodextrins (CDs) have shown great promise as non-viral gene and siRNA delivery vectors in a range of in vitro and in vivo studies. In the current study, structural and biophysical characterisation of selected CDs was carried out to enhance our understanding of their interaction with nucleic acids. The methods used for such characterisation were dynamic light scattering, zeta potential measurements and circular dichroism. Variations in the chemistries of individual CDs and in the type of formulation were shown to affect key properties of complexes such as size, surface charge and nucleic acid conformation. Furthermore, the effects of temperature and pH on the conformation of nucleic acids were investigated. pH studies were intended to mimic the conditions encountered by cationic complexes during endocytosis. Circular dichroism studies revealed that changes occurred in DNA and siRNA conformation upon complexation with CDs and when exposed to increasing temperature and decreasing pH. Overall, siRNA appeared to be more susceptible to conformational changes although complexation of siRNA with CDs tended to have a stabilising effect. (c) 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:1346-1355, 2014
10.1002/jps.23922
Grant Details