O'Connell, KJ,Motherway, MO,Hennessey, AA,Brodhun, F,Ross, RP,Feussner, I,Stanton, C,Fitzgerald, GF,van Sinderen, D

2013

September

Bioengineered

Identification and characterization of an oleate hydratase-encoding gene from Bifidobacterium breve

Validated

WOS: 36 ()

bifidobacteria probiotic conjugated linoleic acid myosin cross reactive antigen hydratase CONJUGATED LINOLEIC-ACID UNSATURATED FATTY-ACIDS CROSS-REACTIVE ANTIGEN OLEIC-ACID LACTOBACILLUS-ACIDOPHILUS PROPIONIBACTERIUM-ACNES 10-HYDROXYSTEARIC ACID GASTROINTESTINAL-TRACT BACTERIA UCC2003

4

313

321

Bifidobacteria are common commensals of the mammalian gastrointestinal tract. Previous studies have suggested that a bifidobacterial myosin cross reactive antigen (MCRA) protein plays a role in bacterial stress tolerance, while this protein has also been linked to the biosynthesis of conjugated linoleic acid (CLA) in bifidobacteria. In order to increase our understanding on the role of MCRA in bifidobacteria we created and analyzed an insertion mutant of the MCRA-encoding gene of B. breve NCFB 2258. Our results demonstrate that the MCRA protein of B. breve NCFB 2258 does not appear to play a role in CLA production, yet is an oleate hydratase, which contributes to bifidobacterial solvent stress protection.

10.4161/bioe.24159