Kenny, OM,McCarthy, CM,Brunton, NP,Hossain, MB,Rai, DK,Collins, SG,Jones, PW,Maguire, AR,O'Brien, NM

2013

April

Life sciences

Anti-inflammatory properties of potato glycoalkaloids in stimulated Jurkat and Raw 264.7 mouse macrophages

Validated

Altmetric: 2 ()

Inflammation Glycoalkaloids Interleukin 2 production Interleukin-8 production Nitric oxide production Jurkat cells Raw 264.7 mouse macrophages NITRIC-OXIDE SYNTHASE INFLAMMATORY-BOWEL-DISEASE NF-KAPPA-B CYTOKINE PRODUCTION ALKALOID GLYCOSIDE CELLS INHIBITION EXPRESSION ADJUVANT TOMATINE

92

775

782

Aims: The potato glycoalkaloids, alpha-chaconine, alpha-solanine and solanidine, along with potato peel extracts were investigated for potential anti-inflammatory effects in vitro. Their potential to reduce two biomarkers of inflammation, cytokine and nitric oxide (NO) productions, were assessed in the stimulated Jurkat and macrophage models, respectively.Main methods: Cytokine and nitric oxide productions were stimulated in Jurkat and Raw 264.7 macrophages with Concanavalin A (Con A; 25 mu g/ml) and lipopolysaccaride (LPS; 1 mu g/ml), respectively. Selective concentrations of glycoalkaloids and potato peel extracts were added simultaneously with Con A or LPS for 24 h to investigate their potential to reduce inflammatory activity.Key findings: alpha-Chaconine and solanidine significantly reduced interleukin-2 (IL-2) and interleukin-8 (IL-8) productions in Con A-induced Jurkat cells. The potato peel extracts did not influence cytokine production. In LPS-stimulated Raw macrophages, alpha-solanine, solanidine and two potato peel extracts significantly reduced induced NO production.Significance: Our findings suggest that sub-cytotoxic concentrations of potato glycoalkaloids and potato peel extracts possess anti-inflammatory effects in vitro and with further investigation may be useful in the prevention of anti-inflammatory diseases. (C) 2013 Elsevier Inc. All rights reserved.

10.1016/j.lfs.2013.02.006