Peer-Reviewed Journal Details
Mandatory Fields
Allocati, N,Masulli, M,Del Boccio, P,Pieragostino, D,D'Antonio, D,Sheehan, D,Di Ilio, C
2014
March
Folia microbiologica
Identification of an elongation factor 1B gamma protein with glutathione transferase activity in both yeast and mycelial morphologies from human pathogenic Blastoschizomyces capitatus
Validated
WOS: 3 ()
Optional Fields
SACCHAROMYCES-CEREVISIAE GLIOTOXIN BIOSYNTHESIS ASPERGILLUS-FUMIGATUS GEOTRICHUM-CAPITATUM YARROWIA-LIPOLYTICA SUPERFAMILY INFECTIONS DIMORPHISM EVOLUTION CLONING
59
107
113
Blastoschizomyces capitatus is an uncommon, opportunistic pathogenic fungus, which causes invasive and disseminated infections. This microorganism is normally present in both environmental and normal human flora. Within a host, B. capitatus is able to grow in both unicellular yeast and multicellular filamentous growth forms. In this study, we obtained in vitro morphological conversion of B. capitatus from yeast-to-mycelial phase to investigate the presence and expression of glutathione transferase (GST) enzymes in both cell forms. A protein with GST activity using the model substrate 1-chloro-2,4-dinitrobenzene was detected in both morphologies and identified by tandem mass spectrometry as a eukaryotic elongation factor 1B gamma (eEF1B gamma) protein, a member of the GST superfamily. No significant difference in GST-specific activity and kinetic constants were observed between mycelial and yeast forms, indicating that eEF1B gamma protein did not show differential expression between the two phases.
10.1007/s12223-013-0273-3
Grant Details