Peer-Reviewed Journal Details
Mandatory Fields
Samatova, E,Konevega, AL,Wills, NM,Atkins, JF,Rodnina, MV
2014
July
Nature Communications
High-efficiency translational bypassing of non-coding nucleotides specified by mRNA structure and nascent peptide
Validated
Optional Fields
OPEN READING FRAMES RIBOSOMAL BYPASS EXIT TUNNEL CODING GAP BACTERIOPHAGE-T4 PROTEINS SEQUENCE GENE-60 FLUORESCENCE INITIATION
5
The gene product 60 (gp60) of bacteriophage T4 is synthesized as a single polypeptide chain from a discontinuous reading frame as a result of bypassing of a non-coding mRNA region of 50 nucleotides by the ribosome. To identify the minimum set of signals required for bypassing, we recapitulated efficient translational bypassing in an in vitro reconstituted translation system from Escherichia coli. We find that the signals, which promote efficient and accurate bypassing, are specified by the gene 60 mRNA sequence. Systematic analysis of the mRNA suggests unexpected contributions of sequences upstream and downstream of the non-coding gap region as well as of the nascent peptide. During bypassing, ribosomes glide forward on the mRNA track in a processive way. Gliding may have a role not only for gp60 synthesis, but also during regular mRNA translation for reading frame selection during initiation or tRNA translocation during elongation.
10.1038/ncomms5459
Grant Details