Kehoe, JJ,Liskova, K,Mok, KH,O'Brien, N,Kelly, AL,Brodkorb, A

2014

September

International Dairy Journal

Formation of cytotoxic alpha-lactalbumin/sodium oleate complexes: Concentration and temperature effects

Validated

WOS: 2 ()

KILLS TUMOR-CELLS OLEIC-ACID BETA-LACTOGLOBULIN FATTY-ACID FLUORESCENCE SPECTROSCOPY BOVINE HAMLET APOPTOSIS PROTEIN LETHAL

38

65

73

Cytotoxic complexes may be formed by mixing whey proteins and oleic acid or sodium oleate. The present study investigated the kinetics of complex formation when the whey protein alpha-lactalbumin (alpha-LA) is mixed with sodium oleate. Intrinsic fluorescence showed that the complex formed at room temperature during the mixing of alpha-LA and sodium oleate. However, some of the structural changes brought about by the binding of the sodium oleate can be reversed by dialysis, when loosely bound oleate is removed. During dialysis, solutions with lower protein concentrations had reduced buffering capacity, and so the pH decreased more; this decrease in pH was correlated with a loss of oleate from the complex. The cytotoxicity of all the samples produced in this study was strongly correlated with the amount of oleate bound in the complex, confirming previous studies that suggested that oleic acid is the cytotoxic agent in the complex. (C) 2014 Elsevier Ltd. All rights reserved.

10.1016/j.idairyj.2014.04.005