Conference Contribution Details
Mandatory Fields
K. O-Donovan, T. Wood, E. Moore, J. Alderman
14th International Worshop on Invitro Toxicology
The use of indium tin oxide as a cell-based biosensor for in vitro analysis
Ostend, Belgium
Poster Presentation
Optional Fields
Cell-based biosensors (CBBs), which treat living cells as sensing elements are able to detect the functional information of biological active analytes and also provide quantificational analysis. In general, they can keep living cells under constant observation to study cellular physiological action when cells are subjected to stimulus and verify the presence and the concentration of these stimuli. CBBs characterise with high sensitivity, excellent selectivity and a fast response time. There has been increased interest in the use of microelectronic biosensors that allow cellular activity to be analysed in vitro. Inter-digitated electrode structures (IDES) have received great attention in the area of electroanalytical chemistry in recent years. IDES have been used for studies of biological cell behaviours with impedance measurements1. Impedance measurements on cellular systems2 with IDES have been shown to be an effective way of monitoring cellular behaviour on-line and in real-time. The IDES sensors are essentially indium tin oxide (ITO) fingers. ITO was used as the impedance sensor and allows real time non-invasive in-vitro analysis of the physiological state of biological cells due to its conductive, biocompatible and transparent characteristics. The neuroblastoma cell line SH-SY5Y was grown on both gold (Au) and ITO IDES to examine cell proliferation, growth and death using bioimpedance measurements obtained using these electrodes. Over the 4-day period studied SH-SY5Y growth/proliferation appeared to be similar to that on T75cm2 control flask of SH-SY5. This inferred that both Au and the ITO IDES were biocompatible over this time. SH-SY5Y cells grown on ITO IDES appeared similar and seemed to retain the characteristic SH-SY5Y shape, than when observed on T75cm2 flask better than that grown on Au IDES. The main effect of cells on IDES is due to the insulating property of the cell membrane. The presence of intact cell membranes on the electrodes determines the current flow and thus the sensor signal3. Growth behaviour, adhesive properties and the physiological state of adherent cells can be monitored during cultivation. The ITO IDES sensor measured the impedance and images were also viewed and photographed using a microscope coupled with a camera.