Peer-Reviewed Journal Details
Mandatory Fields
Kyriakoudi, A,O'Callaghan, YC,Galvin, K,Tsimidou, MZ,O'Brien, NM
2015
October
Journal of Agricultural and Food Chemistry
Cellular Transport and Bioactivity of a Major Saffron Apocarotenoid, Picrocrocin (4-(beta-D-Glucopyranosyloxy)-2,6,6-trimethyl-1-cyclohexene-1-carboxaldehyde)
Validated
WOS: 13 ()
Optional Fields
picrocrocin saffron apocarotenoids crocetin cellular transport bioactivity CROCUS-SATIVUS L. EPITHELIAL CACO-2 CELLS THERMAL-TREATMENT AQUEOUS EXTRACTS DNA-DAMAGE CROCETIN QUERCETIN BIOAVAILABILITY PERMEABILITY MONOLAYERS
63
8662
8668
The cellular transport and bioactivity of the second major saffron apocarotenoid, picrocrocin, was examined in parallel to that of the major group, crocetin sugar esters, in aqueous extracts. The transport of pure picrocrocin was investigated in comparison to that of other saffron apocarotenoids, trans-crocetin (di-beta-D-gentiobiosyl) ester and crocetin using the Caco-2 cell model coupled with an in vitro digestion procedure. RP-HPLC-DAD was employed to quantify the bioaccessible and bioavailable amounts of individual apocarotenoids. Picrocrocin and crocetin sugar esters though highly bioaccessible (75% and 60%, respectively) were transported at minute quantities (0.2% and 0.5%, respectively; 10-fold lower than crocetin). Picrocrocin did not protect against oxidant-induced DNA damage in U937, human monocytic blood cells at the concentration investigated, however, it reduced the proliferation of human adenocarcinoma and hepatocarcinoma cells. Our findings may be useful for the requirements of food legislation regarding saffron preparations, in which both apocarotenoid groups coexist.
10.1021/acs.jafc.5b03363
Grant Details