Peer-Reviewed Journal Details
Mandatory Fields
Collins, B;Curtis, N;Cotter, PD;Hill, C;Ross, RP
2010
October
Antimicrobial Agents and Chemotherapy
The ABC Transporter AnrAB Contributes to the Innate Resistance of Listeria monocytogenes to Nisin, Bacitracin, and Various beta-Lactam Antibiotics
Validated
WOS: 103 ()
Optional Fields
SIGNAL-TRANSDUCTION SYSTEM PENICILLIN-BINDING PROTEIN STAPHYLOCOCCUS-AUREUS BACILLUS-SUBTILIS ANTIMICROBIAL PEPTIDES LACTOCOCCUS-LACTIS LANTIBIOTIC MERSACIDIN GENE-EXPRESSION STRESS-RESPONSE IN-VITRO
54
4416
4423
A mariner transposon bank was used to identify loci that contribute to the innate resistance of Listeria monocytogenes to the lantibiotic nisin. In addition to highlighting the importance of a number of loci previously associated with nisin resistance (mprF, virRS, and telA), a nisin-sensitive phenotype was associated with the disruption of anrB (lmo2115), a gene encoding the permease component of an ABC transporter. The contribution of anrB to nisin resistance was confirmed by the creation of nonpolar deletion mutants. The loss of this putative multidrug resistance transporter also greatly enhanced sensitivity to bacitracin, gallidermin, and a selection of beta-lactam antibiotics. A comparison of the relative antimicrobial sensitivities of a number of mutants established the Delta anrB strain as being one of the most bacitracin-sensitive L. monocytogenes strains identified to date.
WASHINGTON
0066-4804
10.1128/AAC.00503-10
Grant Details