Margassery, LM;Kennedy, J;O'Gara, F;Dobson, AD;Morrissey, JP

2012

January

Journal of Microbiological Methods

A high-throughput screen to identify novel calcineurin inhibitors

Validated

Altmetric: 2 ()

CYCLOSPORINE-A YEAST-CELLS STRESS FK-506 FUNGI ASSAY

88

63

66

Calcineurin is a eukaryotic protein phosphatase important for many signalling and developmental processes in cells. Inhibitors of this enzyme are used clinically and there is interest in identifying novel inhibitors for therapeutic applications. This report describes a high-throughput assay that can be used to screen natural or chemical libraries of compounds to identify new calcineurin inhibitors. The microtitre plate assay is based on a yeast reporter strain and was validated with known inhibitors and tested in a pilot screen of bacterial extracts. (C) 2011 Elsevier B.V. All rights reserved.

AMSTERDAM

0167-7012

10.1016/j.mimet.2011.10.012