Peer-Reviewed Journal Details
Mandatory Fields
McCarthy, AL;O'Callaghan, YC;Connolly, A;Piggott, CO;FitzGerald, RJ;O'Brien, NM
2013
November
Food & Function
Brewers' spent grain (BSG) protein hydrolysates decrease hydrogen peroxide (H2O2)-induced oxidative stress and concanavalin-A (con-A) stimulated IFN-gamma production in cell culture
Validated
WOS: 11 ()
Optional Fields
ANTIOXIDATIVE PEPTIDES POTENTIAL APPLICATIONS ENZYMATIC-HYDROLYSIS PHENOLIC EXTRACTS IN-VITRO INFLAMMATION NUTRITION ACID
4
1709
1716
The present study investigated the bioactivity of protein hydrolysates and fractionated hydrolysates prepared from brewers' spent grain (BSG) using proteases, including Alcalase 2.4L, Flavourzyme and Corolase PP. Hydrolysates were designated K-Y, including fractionated hydrolysates with molecular weight (m.w.) < 3, <5 and >5 kDa. Where computable, IC50 values were lower in U937 (1.38-9.78%) than Jurkat T cells (1.15-13.82%). Hydrolysates L, Q and R and fractionated hydrolysates of U and W (<3, <5, >5 kDa) significantly (P < 0.01) protected against hydrogen peroxide (H2O2)-induced reduction of superoxide dismutase (SOD) activity. A fractionated hydrolysate of W (<5 kDa) protected against H2O2-induced DNA damage, P < 0.01. Hydrolysates K, N, P, U, U > 5 kDa, V, V > 5 kDa, W, W > 5 kDa significantly (P < 0.05) reduced a concanavlin-A (con-A) stimulated production of interferon-gamma (IFN-gamma). In conclusion, BSG protein hydrolysates demonstrate bioactivity in vitro; lower m.w. hydrolysates (<3, <5 kDa) show greatest antioxidant activity and unfractionated or higher m.w. hydrolysates (>5 kDa) possess anti-inflammatory effects.
CAMBRIDGE
2042-6496
10.1039/c3fo60191a
Grant Details