O'Sullivan, A;Gibney, MJ;Connor, AO;Mion, B;Kaluskar, S;Cashman, KD;Flynn, A;Shanahan, F;Brennan, L

2011

May

Molecular Nutrition & Food Research

Biochemical and metabolomic phenotyping in the identification of a vitamin D responsive metabotype for markers of the metabolic syndrome

Validated

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GESTATIONAL DIABETES-MELLITUS APOLIPOPROTEIN-A-I INSULIN-RESISTANCE D DEFICIENCY 25-HYDROXYVITAMIN D GLUCOSE-TOLERANCE D SUPPLEMENTATION HYPOVITAMINOSIS-D US ADULTS HEALTH

55

679

690

Scope: Metabolic phenotyping promises to be a useful tool in human intervention studies. This study examined whether metabolic phenotyping could identify responders to vitamin D supplementation in terms of the metabolic syndrome. Methods and results: In a double-blind, randomised placebo-controlled dietary intervention subjects were assigned to receive 15 mu g vitamin D(3) or placebo daily. Serum 25-hydroxyvitamin D (25(OH)D) and biochemical markers of the metabolic syndrome were measured at baseline and following the 4-wk intervention. k-means clustering and (1)H-NMR metabolomic analysis were used to explore responsive phenotypes. Vitamin D supplementation significantly increased serum 25(OH) D to an endpoint concentration of 78.1 +/- 20.0 nmol/L (p < 0.001). There was no effect of supplementation on the measured markers of the metabolic syndrome. k-means cluster analysis based on 13 biochemical markers of the metabolic syndrome and 25(OH)D concentrations revealed five discrete biomarker clusters. One of these clusters, characterised by lower serum 25(OH) D and higher levels of adipokines, showed significant responses in insulin (15% decrease), homestatic model assessment scores (19% decrease) and c-reactive protein (54% decrease). Metabolomic analysis revealed further changes and the extent of change in serum vitamin D correlated negatively with changes in glucose. Conclusion: Overall, metabolic phenotyping revealed a phenotype that was responsive to vitamin D supplementation.

MALDEN

1613-4125

10.1002/mnfr.201000458