Peer-Reviewed Journal Details
Mandatory Fields
Villari, V;Mazzaglia, A;Darcy, R;O'Driscoll, CM;Micali, N
2013
March
Biomacromolecules
Nanostructures of Cationic Amphiphilic Cyclodextrin Complexes with DNA
Validated
WOS: 47 ()
Optional Fields
GENE DELIVERY-SYSTEMS SIRNA DELIVERY STRUCTURAL-PROPERTIES BILAYER VESICLES LIGHT-SCATTERING NANOASSEMBLIES NANOPARTICLES SURFACTANT VECTORS PROTEIN
14
811
817
Complexes of cationic amphiphilic cyclodextrins heptakis[2-(omega-amino-oligo(ethylene glycol))-6-deoxy-6-hexadecylthio]-beta-cyclodextrin and heptakis[2-(omega-amino-oligo(ethylene glycol))-6-deoxy-6-dodecylthio]-beta-cyclodextrin with DNA were examined by small-angle X-ray scattering and dynamic as well as electrophoretic light scattering. The first cyclodextrin forms bilayer vesicles in water, which, in the presence of calf thymus DNA, transform to a multilamellar complex. In this complex, the DNA lies between the two polar layers of the cyclodextrins protonated amino groups in alternation with the lipidic bilayers. The cyclodextrin with shorter lipid chains, in contrast, forms micelles in water, and electrostatic clustering of these about DNA does not affect their intrinsic structure. These results are relevant to the potential of such cyclodextrins in therapeutic gene delivery, showing that their self-assembly modes in isolation influence their complex formation with DNA and possibly their efficiency in promoting cell transfection.
WASHINGTON
1525-7797
10.1021/bm3018609
Grant Details