Peer-Reviewed Journal Details
Mandatory Fields
O'Callaghan, YC;Woods, JA;O'Brien, NM
2001
January
Cell Biology and Toxicology
Comparative study of the cytotoxicity and apoptosis-inducing potential of commonly occurring oxysterols
Validated
WOS: 58 ()
Optional Fields
CHOLESTEROL OXIDATION-PRODUCTS LOW-DENSITY-LIPOPROTEIN ENDOTHELIAL-CELLS ANTIOXIDANT DEFENSE GSH EXTRUSION GLUTATHIONE INHIBITION OXIDES MONOLAYERS MECHANISMS
17
127
137
The cytotoxicity of the oxysterols 25-hydroxycholesterol, 7 beta -hydroxycholesterol, cholesterol-5 alpha ,6 alpha -epoxide, cholesterol-5 beta ,6 beta -epoxide, 19-hydroxycholesterol and 7-ketocholesterol was examined in U937 cells, a human monocytic blood cell line. 7 beta -Hydroxycholesterol, cholesterol-5 beta ,6 beta -epoxide, and 7-ketocholesterol, at 30 mu mol/L concentration, were found to be cytotoxic to this cell line and the mode of cell death was by apoptosis. 25-Hydroxycholesterol, cholesterol-5 alpha ,6 alpha -epoxide and 19-hydroxycholesterol (30 mu mol/L) did not induce apoptosis in this cell line. Since it has been suggested that the generation of an oxidative stress may occur in the early stages of the apoptotic process, the glutathione concentration and the activity of superoxide dismutase were also measured in the oxysterol-treated cells. 7 beta -Hydroxycholesterol was shown to increase the superoxide dismutase activity and decrease the glutathione concentration. However, cholesterol-5 beta ,6 beta -epoxide and 7-ketocholesterol, which were also shown to induce apoptosis, did not affect the glutathione concentration or the superoxide dismutase activity in the U937 cells. Therefore, oxysterol-induced apoptosis may not be dependent on the generation of an oxidative stress.
DORDRECHT
0742-2091
Grant Details