Peer-Reviewed Journal Details
Mandatory Fields
Higgins, S;Carroll, YL;McCarthy, SN;Corridan, BM;Roche, HM;Wallace, JMW;O'Brien, NM;Morrissey, PA
2001
January
British Journal of Nutrition
Susceptibility of LDL to oxidative modification in healthy volunteers supplemented with low doses of n-3 polyunsaturated fatty acids
Validated
WOS: 43 ()
Optional Fields
LOW-DENSITY-LIPOPROTEIN FISH-OIL SUPPLEMENTATION VITAMIN-E DOCOSAHEXAENOIC ACID LIPID-PEROXIDATION DIETARY SUPPLEMENTATION HYPERTRIGLYCERIDEMIC PATIENTS EICOSAPENTAENOIC ACID IN-VITRO PLASMA
85
23
31
The objective of the present study was to evaluate the oxidative susceptibility of LDL in human volunteers following supplementation with various low doses (<1 g/d) of n-3 polyunsaturated fatty acids (PUFA). Sixty-two healthy volunteers (thirty-seven males and twenty-five females, aged 19-63 years) were recruited to take part in a randomised placebo-controlled trial. Volunteers were required to take 0.9, 0.6 or 0.3 g n-3 PUFA as fish oil or placebo capsules daily for 16 weeks. Susceptibility of LDL to oxidative modification was assessed by measuring the production of conjugated dienes and thiobarbituric acid-reactive substances in LDL oxidised by Cu(2+) (15 M) or 2,2'-azobis(2-amidinopropane) dihydrochloride (1 mM) for 5 h. Plasma fatty acid and LDL-fatty acid composition, cholesterol levels and antioxidant concentrations were also measured. While post-treatment n-3 PUFA compositions of plasma and LDL reflected the capsule contents, no meaningful differences in antioxidant concentrations or cholesterol levels were observed between the groups. Supplementation with low doses of n-3 PUFA as fish oil did not influence the oxidative susceptibility of LDL. The results of the present study suggest that moderate dietary intakes of n-3 PUFA do not significantly influence the susceptibility of LDL to oxidative modification in vitro.
CAMBRIDGE
0007-1145
Grant Details