Peer-Reviewed Journal Details
Mandatory Fields
Simpson, PJ;Fitzgerald, GF;Stanton, C;Ross, RP
2006
January
Journal of Microbiological Methods
Enumeration and identification of pediococci in powder-based products using selective media and rapid PFGE
Validated
WOS: 18 ()
Optional Fields
ANTIMICROBIAL SUSCEPTIBILITY ANIMAL FEED BIFIDOBACTERIUM RESISTANT VANCOMYCIN AGENTS
64
120
125
In this study, pediococci selective medium (PSM) was evaluated for the enumeration of Pediococcus acidilactici and Pediococcus pentosaceus from probiotic animal feed and silage inoculants. PSM is based on the complex basal medium MRS supplemented with cysteine hydrochloride, novobiocin, vancomycin, and nystatin. No significant change in electivity was observed when pediococci where recovered from culture or powder-based products following incubation at 37 degrees C under anaerobic conditions for 24 h. The medium was suitable for the enumeration of pediococci in samples also containing bacilli, bifidobacteria, enterococci, lactobacilli, lactococci, propionibacteria, streptococci, and yeast components. However, to inhibit Lactobacillus plantarum and Lactobacillus casei, ampicillin was added and the revised medium, termed PSM+A, was also considered to be suitably elective for pediococci recovered from powder. In addition, a rapid PFGE protocol is presented, which allows Pediococcus species and strain verification from colonies in less than 3 days. (c) 2005 Elsevier B.V. All rights reserved.
AMSTERDAM
0167-7012
10.1016/j.mimet.2005.04.019
Grant Details