Peer-Reviewed Journal Details
Mandatory Fields
Trotter, M;McAuliffe, O;Callanan, M;Edwards, R;Fitzgerald, GF;Coffey, A;Ross, RP
2006
January
Gene
Genome analysis of the obligately lytic bacteriophage 4268 of Lactococcus lactic provides insight into its adaptable nature
Validated
WOS: 18 ()
Optional Fields
STREPTOCOCCUS-THERMOPHILUS MOLECULAR CHARACTERIZATION TEMPERATE BACTERIOPHAGE SEQUENCE-ANALYSIS HOMOLOGOUS RECOMBINATION VIRULENT BACTERIOPHAGE PROHEAD PROTEASE PHAGE RESISTANCE DNA-REPLICATION PLASMID PTR2030
366
189
199
Analysis of the complete nucleotide sequence of the lactococcal phage 4268, which is lytic for the cheese starter Lactococcus lactis DPC4268, is presented. Phage 4268 has a linear genome of 36,596 bp, which is modularly organised and encompasses 49 open reading frames. Putative functions were assigned to approximately 45% of the predicted products of these open reading frames based on sequence similarity with known proteins, N-terminal sequence analysis and identification of conserved domains. Significantly, a segment of the genome has homology to the recently sequenced lysogenic module in lactococcal phage phi 31 that contains a lytic switch but no phage integrase or attachment site. This suggests that it is derived from a prophage. A phage 4268-encoded and a host-encoded methylase were found to be highly similar, having only two nucleotide mismatches, suggesting that the phage acquired the methylase gene to protect it from a host endonuclease. Comparative genomic analysis revealed significant homology between phage 4268 and the lactococcal phage BK5-T. The comparative analysis also supported the classification of phage 4268 and other BK5-T-related phage as separate from the proposed P335 species of lactococcal phage. (c) 2005 Elsevier B.V. All rights reserved.
AMSTERDAM
0378-1119
10.1016/j.gene.2005.09.022
Grant Details