Peer-Reviewed Journal Details
Mandatory Fields
Watson, D;Sleator, RD;Hill, C;Gahan, CGM
2008
October
BMC Microbiology
Enhancing bile tolerance improves survival and persistence of Bifidobacterium and Lactococcus in the murine gastrointestinal tract
Validated
WOS: 58 ()
Optional Fields
LACTOBACILLUS-SALIVARIUS UCC118 PATHO-BIOTECHNOLOGY APPROACH CONTROLLED GENE-EXPRESSION LISTERIA-MONOCYTOGENES INTESTINAL MICROBIOTA PROBIOTIC RESEARCH BREVE UCC2003 BAD BUGS LACTIS INTERLEUKIN-10
8
Background: The majority of commensal gastrointestinal bacteria used as probiotics are highly adapted to the specialised environment of the large bowel. However, unlike pathogenic bacteria; they are often inadequately equipped to endure the physicochemical stresses of gastrointestinal (GI) delivery in the host. Herein we outline a patho-biotechnology strategy to improve gastric delivery and host adaptation of a probiotic strain Bifidobacterium breve UCC2003 and the generally regarded as safe (GRAS) organism Lactococcus lactis NZ9000. Results: In vitro bile tolerance of both strains was significantly enhanced (P < 0.001), following heterologous expression of the Listeria monocytogenes bile resistance mechanism BilE. Strains harbouring bilE were also recovered at significantly higher levels (P < 0.001), than control strains from the faeces and intestines of mice (n = 5), following oral inoculation. Furthermore, a B. breve strain expressing bilE demonstrated increased efficacy relative to the wild-type strain in reducing oral L. monocytogenes infection in mice. Conclusion: Collectively the data indicates that bile tolerance can be enhanced in Bifidobacterium and Lactococcus species through rational
LONDON
1471-2180
10.1186/1471-2180-8-176
Grant Details