Peer-Reviewed Journal Details
Mandatory Fields
Russo, A;MoenneLoccoz, Y;Fedi, S;Higgins, P;Fenton, A;Dowling, DN;ORegan, M;OGara, F
1996
February
Applied Microbiology and Biotechnology
Improved delivery of biocontrol Pseudomonas and their antifungal metabolites using alginate polymers
Validated
WOS: 30 ()
Optional Fields
LACTIS SUBSP LACTIS FLUORESCENS CELLS ENTRAPPED CELLS SURVIVAL BACTERIA GELS SOIL 2,4-DIACETYLPHLOROGLUCINOL IMMOBILIZATION COLONIZATION
44
740
745
Alginate polymer was evaluated as a carrier for seed inoculation with a genetically modified strain Pseudomonas fluorescens F113LacZY, which protects sugar-beet against Pythium-mediated damping-off. F113LacZY survived in alginate beads at 5 log(10) CFU/bead or higher counts for 8 weeks of storage, regardless of the conditions of incubation. In plant inoculation experiments, colonisation of the growing area of the root by F113LacZY, derived from alginate beads placed in the soil next to the seed or from an alginate coating around the seeds, was improved compared with application of just free cells of the strain. F113LacZY trapped in alginate beads was an effective producer of antifungal phloroglucinols as indicated by direct HPLC quantification of phloroglucinols and in vitro inhibition of both the indicator bacterium Bacillus Subtilis A1 and the pathogenic fungus Pythium ultimum. Alginate polymer represents a promising carrier for the delivery of biocontrol inoculants for root colonisation and production of antifungal metabolites.
NEW YORK
0175-7598
Grant Details