Peer-Reviewed Journal Details
Mandatory Fields
Corr, S;Hill, C;Gahan, CGM
2006
December
Microbial Pathogenesis
An in vitro cell-culture model demonstrates internalin- and hemolysin-independent translocation of Listeria monocytogenes across M cells
Validated
WOS: 37 ()
Optional Fields
INTESTINAL M-CELLS MURINE PEYERS-PATCHES SALMONELLA-TYPHIMURIUM MAMMALIAN-CELLS TRANSEPITHELIAL TRANSPORT INTRACELLULAR GROWTH ADHERENCE HOST PENETRATION ENTEROCYTES
41
241
250
An ability to translocate the mucosal epithelia through M cells provides invasive pathogens with a rapid means of accessing the mucosal lymphoid tissues. In order to determine the role of M cells in Listeria inonocytogenes infection, we initially assessed colonization of Peyer's patch (PP) epithelium in BALB/c mice by Vibrio cholerae Eltor, wild-type L. monocytogenes and an isogenic hemolysin mutant (LO28 Delta hly). It was observed that both wild-type L. monocytogenes and Delta hly showed preferential colonization of PP epithelium in this model. Furthermore, a novel luciferase reporter system was used to show rapid site-specific localization of L. monocytogenes in intestinal Peyer's patches. To examine the role of M cells in transcytosis of L. monocytogenes we utilized an in vitro transwell model that mimics M-cell activity through differentiation of C2Bbel epithelial enterocytes via co-culture with murine Peyer's patch lymphocytes (PPL). It was shown that L. monocytogenes transits M cells at significantly increased rates compared to C2Bbel monocultures. In addition, M-cell transport occurred independently of bacterial hemolysin and internalin production. This study demonstrates rapid transcytosis of L. monocytogenes through M cells, a process that occurs independently of the action of classical virulence factors. (c) 2006 Elsevier Ltd. All rights reserved.
LONDON
0882-4010
10.1016/j.micpath.2006.08.003
Grant Details