Peer-Reviewed Journal Details
Mandatory Fields
Kenny, O;O'Callaghan, Y;O'Connell, NM;McCarthy, FO;Maguire, AR;O'Brien, NM
2012
June
Journal of Agricultural and Food Chemistry
Oxidized Derivatives of Dihydrobrassicasterol: Cytotoxic and Apoptotic Potential in U937 and HepG2 Cells
Validated
WOS: 14 ()
Optional Fields
CHOLESTEROL OXIDATION-PRODUCTS LIVER-X-RECEPTORS PHYTOSTEROL OXIDES BETA-SITOSTEROL DEATH 7-BETA-HYDROXYCHOLESTEROL CHOLESTEROL-5,6-EPOXIDES STIGMASTEROL OXYSTEROLS MECHANISMS
60
5952
5961
The ability of phytosterol compounds to reduce plasma serum cholesterol levels in humans is well investigated. However, phytosterols are structurally similar to cholesterol with a double bond at the C5-6 position and are therefore susceptible to oxidation. Much research has been carried out on the biological effects of cholesterol oxidation products (COPs) in vitro. In contrast, there is less known about phytosterol oxidation products (POPs). From previous studies, it is apparent that oxidized derivatives of the phytosterols, beta-sitosterol and stigmasterol, are cytotoxic in vitro but are less potent than their COP counterparts. In the present study, the cytotoxic and apoptotic potential of oxidized derivatives of dihydrobrassicasterol (DHB) including 5 alpha,6 alpha-epoxyergostan-3 beta-ol (alpha-epoxide), 5 beta,6 beta-epoxyergostan-3 beta-ol (beta-epoxide), ergost-5-en-7-on-3 beta-ol (7-keto), ergost-5-ene-3 beta,7 beta-diol (7-beta-OH), and ergostane-3 beta,5 alpha,6 beta-triol (triol) were evaluated in the U937 and HepG2 cell lines. In general, 7-keto, 7-beta-OH, and triol derivatives had a significant cytotoxic impact on U937 and HepG2 cells. The oxides appear to be more toxic toward U937 cells. In line with previous findings, the POPs investigated in this study were less potent than the equivalent COPs. The results add to the body of data on the toxicity of individual POPs.
WASHINGTON
0021-8561
10.1021/jf204737e
Grant Details