Peer-Reviewed Journal Details
Mandatory Fields
Clover, AJP,Kumar, AHS,Isakson, M,Whelan, D,Stocca, A,Gleeson, BM,Caplice, NM
2015
May
Journal of The International Society For Burn Injuries
Allogeneic mesenchymal stem cells, but not culture modified monocytes, improve burn wound healing
Validated
WOS: 31 ()
Optional Fields
Mesenchymal stem cells Myeloid cells Cell therapy Burn wound healing ENDOTHELIAL PROGENITOR CELLS BLOOD MONOCYTES PORCINE MODEL SKIN FIBRIN INJURY NEOVASCULARIZATION DIFFERENTIATION ANGIOGENESIS MOBILIZATION
41
548
557
The use of cell therapy to improve burn wound healing is limited as a validated cell source is not rapidly available after injury. Progenitor cells have shown potential to drive the intrinsic wound regeneration.Two sources of cells, allogeneic mesenchymal stem cells (MSC) and autologous culture modified monocytes (CMM), were assessed for their ability to influence burn wound healing. Both could be widely available shortly after injury. Cells were delivered in a fibrin matrix following contact burns in a porcine burns model.Application of MSC significantly decreased the area of unhealed burn compared to CMM or delivery matrix alone (6% MSC, 27% CMM, 24% Matrix, p < 0.001). MSC treated wounds showed histological evidence of improved wound healing with increased collagen content (MSC 49%, CMM 42%, p < 0.01), increased epidermal area (MSC 8.8%, CMM 6.1%, p < 0.01) and dermal thickness (MSC 1108 mu m, CMM 1007 mu m, p < 0.01) compared to CMM treated wounds. Labelled MSC and CMM were identified in the wounds after 2 weeks by immunohistochemistry and FACS.A single application of allogeneic MSC improves the rate of burn wound healing and improves the histological appearance of the burn wound. These cells show potential as a cell therapy that is rapidly available following burn. (C) 2014 Elsevier Ltd and ISBI. All rights reserved.
10.1016/j.burns.2014.08.009
Grant Details