Peer-Reviewed Journal Details
Mandatory Fields
Moore, A.,McGuirk, P.,Adams, S.,Jones, W. C.,McGee, J. P.,O'Hagan, D. T.,Mills, K. H.
Immunization with a soluble recombinant HIV protein entrapped in biodegradable microparticles induces HIV-specific CD8+ cytotoxic T lymphocytes and CD4+ Th1 cells
Optional Fields
Animals Antibody Specificity Biocompatible Materials Biodegradation CD8-Positive T-Lymphocytes/*immunology Cell Division/immunology Cytotoxicity, Immunologic Drug Carriers HIV Envelope Protein gp120/administration & dosage/*immunology *Immunization *Lactic Acid Mice Mice, Inbred BALB C Microspheres *Polyglycolic Acid Polymers Research Support, Non-U.S. Gov't Solubility Th1 Cells/*immunology
One of the major obstacles to the development of successful recombinant vaccines against human immunodeficiency virus (HIV) and other intracellular pathogens is the identification of a safe and effective vaccine delivery system for the induction of cell mediated immunity with soluble protein antigens. In this study it was demonstrated that immunization with a recombinant HIV envelop (env) protein entrapped in biodegradable poly(lactide-co-glycolide) (PLG) microparticles induced consistent HIV-specific CD4+ and CD8+ T-cell responses in mice. Major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes (CTL) responses were detected following a single systemic immunization with gp120 entrapped microparticles and when given by the intranasal (i.n.) route induced HIV-specific CD8+ CTL and secretory IgA. Furthermore immunization with gp120 entrapped in microparticles generated CD4+ T cells that secreted moderate to high levels of IFN-gamma. Therefore, PLG microparticles are a safe and effective means of delivering antigen to the appropriate processing site for the generation of class I-restricted CTL, and are also capable of inducing Th1 cells.
Grant Details