Book Chapter Details
Mandatory Fields
Anastasia Shpichka, Anastasia Koroleva, Daria Kuznetsova, Ruslan I. Dmitriev, Peter Timashev
2017 Unknown
Multi-Parametric Live Cell Microscopy of 3D Tissue Models
Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues
Springer International Publishing AG 2017
Springer, Cham
Published
0
Optional Fields
Decellularization, Fibrin gel, Microscopy, MTT-assay, Scaffolds, Two-photon polymerization
Polymeric, ceramic and hybrid material-based three-dimensional (3D) scaffold or matrix structures are important for successful tissue engineering. While the number of approaches utilizing the use of cell-based scaffold and matrix structures is constantly growing, it is essential to provide a framework of their typical preparation and evaluation for tissue engineering. This chapter describes the fabrication of 3D scaffolds using two-photon polymerization, decellularization and cell encapsulation methods and easy-to-use protocols allowing assessing the cell morphology, cytotoxicity and viability in these scaffolds.
Dr. R. Dmitriev
978-3-319-67357-8
https://link.springer.com/chapter/10.1007/978-3-319-67358-5_5
71
81
https://doi.org/10.1007/978-3-319-67358-5_5
Grant Details