Book Chapter Details
Mandatory Fields
Anastasia Shpichka, Anastasia Koroleva, Daria Kuznetsova, Ruslan I. Dmitriev, Peter Timashev
2017 Unknown
Multi-Parametric Live Cell Microscopy of 3D Tissue Models
Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues
Springer International Publishing AG 2017
Springer, Cham
Optional Fields
Decellularization, Fibrin gel, Microscopy, MTT-assay, Scaffolds, Two-photon polymerization
Polymeric, ceramic and hybrid material-based three-dimensional (3D) scaffold or matrix structures are important for successful tissue engineering. While the number of approaches utilizing the use of cell-based scaffold and matrix structures is constantly growing, it is essential to provide a framework of their typical preparation and evaluation for tissue engineering. This chapter describes the fabrication of 3D scaffolds using two-photon polymerization, decellularization and cell encapsulation methods and easy-to-use protocols allowing assessing the cell morphology, cytotoxicity and viability in these scaffolds.
Dr. R. Dmitriev
Grant Details