Peer-Reviewed Journal Details
Mandatory Fields
Cummins, J.,Reen, F. J.,Baysse, C.,Mooij, M. J.,O'Gara, F.
Subinhibitory concentrations of the cationic antimicrobial peptide colistin induce the pseudomonas quinolone signal in Pseudomonas aeruginosa
WOS: 51 ()
Optional Fields
Anti-Bacterial Agents/*administration & dosage Colistin/*administration & dosage DNA, Bacterial/genetics/metabolism Dose-Response Relationship, Drug Gene Expression Profiling Gene Expression Regulation, Bacterial/drug effects Genes, Bacterial Humans Hydroxyquinolines/metabolism Oligonucleotide Array Sequence Analysis Pseudomonas Infections/drug therapy/metabolism Pseudomonas aeruginosa/*drug effects/genetics/*metabolism Pyocyanine/biosynthesis Quinolones/*metabolism Quorum Sensing *Transcriptional Activation
Pt 9
Colistin is an important cationic antimicrobial peptide (CAMP) in the fight against Pseudomonas aeruginosa infection in cystic fibrosis (CF) lungs. The effects of subinhibitory concentrations of colistin on gene expression in P. aeruginosa were investigated by transcriptome and functional genomic approaches. Analysis revealed altered expression of 30 genes representing a variety of pathways associated with virulence and bacterial colonization in chronic infection. These included response to osmotic stress, motility, and biofilm formation, as well as genes associated with LPS modification and quorum sensing (QS). Most striking was the upregulation of Pseudomonas quinolone signal (PQS) biosynthesis genes, including pqsH, pqsB and pqsE, and the phenazine biosynthesis operon. Induction of this central component of the QS network following exposure to subinhibitory concentrations of colistin may represent a switch to a more robust population, with increased fitness in the competitive environment of the CF lung.
1350-0872 (Print) 1350-08
Grant Details