Peer-Reviewed Journal Details
Mandatory Fields
Culligan, EP;Sleator, RD;Marchesi, JR;Hill, C
2014
July
Plos One
Metagenomic Identification of a Novel Salt Tolerance Gene from the Human Gut Microbiome Which Encodes a Membrane Protein with Homology to a brp/blh-Family beta-Carotene 15,15'-Monooxygenase
Validated
WOS: 19 ()
Optional Fields
ESCHERICHIA-COLI LISTERIA-MONOCYTOGENES STRESS-RESPONSE GASTROINTESTINAL-TRACT STAPHYLOCOCCUS-AUREUS SEQUENCE ALIGNMENTS BACTERIAL STRESS OXIDATIVE STRESS BINDING-PROTEIN SIGMA-FACTOR
9
The human gut microbiome consists of at least 3 million non-redundant genes, 150 times that of the core human genome. Herein, we report the identification and characterisation of a novel stress tolerance gene from the human gut metagenome. The locus, assigned brpA, encodes a membrane protein with homology to a brp/blh-family beta-carotene monooxygenase. Cloning and heterologous expression of brpA in Escherichia coli confers a significant salt tolerance phenotype. Furthermore, when cultured in the presence of exogenous beta-carotene, cell pellets adopt a red/orange pigmentation indicating the incorporation of carotenoids in the cell membrane.
SAN FRANCISCO
1932-6203
10.1371/journal.pone.0103318
Grant Details